Saotome is the homeroom teacher for class 1-B and is also the physical education teacher. He is often highly energetic and enthusiastic, but due to this he also often makes a scene in front of his students. He is almost never seen without his jersey. Rebecca once teased him about the fact that "it must be great to be a PE teacher and see high school girls in their gym uniforms all the time" trying to imply some other side of Saotome's personality, though this is never confirmed.
Class 1-B
Suzune Shiratori
Suzune is a tall, athletic girl and the best friend of Otome Akiyama. Suzune's favorite hobby is to put Otome in a hold and press on a spot on her head, saying that this will keep Otome, who is very sensitive about her short stature, from growing taller, though instead of hitting the pressure point to stunt her growth, always hits the pressure point to induce diarrhea. When she is angry or annoyed her hand mysteriously becomes larger and she lets loose a devastating "chop" on an unsuspecting character who is always left incapacitated afterward. This furthers the fact that she is a very strong girl even though it doesn't look it at first.
Later in the anime series she shows a talent for making hand puppets depicting chibi forms of some of the characters, herself included.
Otome Akiyama
Otome is very athletic, especially known for her track and field abilities. Her short height becomes a running joke in the series but Suzune is the one that teases her about it most of the time. In fact, she hasn't grown an inch since she was in junior high school, which when she makes this comment, would have been about nine months. She even comes to Becky late in the series asking for help in this field, but Becky can't help her. Also, she is often disrespectful to her superiors, especially Saotome, refusing to refer to him as "sensei" (先生, "sensei"? meaning "teacher").
Hibiki Watanuki
Hibiki is the manager of the school council Intelligence Club and is constantly assuming undercover activities. She always carries a camcorder to record school events with her two subordinates Akira and Yuzuko, especially those concerning Becky. When doing this, she will often change her mind about what to report on, always wanting to find a better "scoop". She considers Rei to be her rival.
Yankee
Yankee usually makes very brief appearances, portrayed as a nerdy, fat character. He hates physical education class and frequently skips that class. He also frequently adds "dot com" to everything he says (for example, "He's totally lost it, dot com.") "Yankee" in Japanese slang refers to rebellious high school students known for dying their hair and engaging in anti-social behavior. In a few times in the anime, he is shown with a strange purple snake coming out of his mouth which even speaks in one episode.
Zoola
According to the "Official Guidebook" sold in Japan, Zoola is a girl, though she looks like a guy. Because she is from some foreign country, her Japanese is not so fluent. She sometimes appears with a curious costume inspired from the classic manga of Yasuzi Tanioka, saying "Asa nanoyo" (朝ナノヨ!, "Asa nanoyo"? literally meaning, "Morning comes!").
Itō
Not much is known or revealed about this character in the animated series. She wears the standard Peach Moon uniform and her face is drawn differently than the other characters in that she has a bulbous nose, fat lips and pitch black eyes. Multiple Itō's are often used to represent crowds of people or the other members of class 1-B.
It is shown in one scene in the anime where she possibly died and was reborn after several cats started to feast on her corpse, not unlike that of Catwoman.